Associate Professor John Hunt

Fields of research: Humoral Immunology and Immunochemistry

Contact Details

Phone: +61 2 9385 1580
Fax: 51389
Email: j.hunt@unsw.edu.au

Tabs

Main

Research Interests:
My research interests focus on mast cell biology and the discovery and molecular characterisation of novel mast cell proteases. We have recently discovered a number of novel human mast cell tryptases, and we are currently determining their substrate specificity. Another aspect of our work involves the characterisation of factors that control the biology of mast cells. We have demonstrated that IL-15 is one of the most potent mast cell attractants yet reported, and also acts to upregulate the expression of ubiquitin-protein ligase 3B, matrix metalloproteinase 9 (MMP9) and apolipoprotein CI (ApoC1) by mast cells.

Broad Research Areas:
Allergy, Biochemistry, Genetics - Genome Analysis, Immunology

Qualifications:
BSc Hons Syd, PhD UNSW

Society Memberships & Professional Activities:
ASMR

Specific Research Keywords:
Inflammation, Protease, Genome search, mast cell biology, Tryptase

University role:

Deputy Head of School

Groups:

Arthritis Research Group

Research

Socio-economic Objective (SEO) tags:

Immune System and Allergy

Publications related to this Person

Book Chapters

Hunt JE; Stevens RL, 1996, 'Mouse mast cell proteases', in Biological and Molecular aspects of Mast Cell and Basophil, Differentiation and Function, edn. Original, Raven Press, New York, USA, pp. 149 - 160

Hunt J; Krilis, 1994, 'Immunology of Antiphospholipid Antibodies', in Panayi GS (ed.), Immunology of the Connective Tissue Diseases, Kluwer Academic Pub

Journal articles

Yang Z; Yan WX; Cai H; Tedla N; Armishaw C; Di Girolamo N; Wang HW; Hampartzoumian T; Simpson JL; Gibson PG, 2007, 'S100A12 provokes mast cell activation: A potential amplification pathway in asthma and innate immunity', Journal of Allergy and Clinical Immunology, vol. 119, pp. 106 - 114, http://dx.doi.org/10.1016/j.jaci.2006.08.021

Hettiaratchi A; Hawkins NJ; McKenzie G; Ward RL; Hunt JE; Wakefield D; Di Girolamo N, 2007, 'The collagenase-1 (MMP-1) gene promoter polymorphism - 1607/2G is associated with favourable prognosis in patients with colorectal cancer', British Journal of Cancer, vol. 96, pp. 783 - 792, http://dx.doi.org/10.1038/sj.bjc.6603630

Geczy CL; Yang Z; Yan W-X; Cai H; Tedla N; Perry M; Hunt J; Hart P, 2005, 'S100A12 and mast cell activation in inflammation', INFLAMMATION RESEARCH, vol. 54, pp. S129 - S129, http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000205501600134&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a

Wei XM; Kim HS; Kumar RK; Heywood GJ; Hunt JE; McNeil HP; Thomas PS, 2005, 'Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cells', RESPIRATORY RESEARCH, vol. 6, http://dx.doi.org/10.1186/1465-9921-6-108

Jackson NE; Wang H-W; Tedla N; McNeil HP; Geczy CL; Collins A; Grimm MC; Hampartzoumian T; Hunt JE, 2005, 'IL-15 induces mast cell migration via a pertussis toxin-sensitive receptor', European Journal of Immunology, vol. 35, pp. 2376 - 2385, http://dx.doi.org/10.1002/eji.200526154

Wang H-W; Tedla N; Hunt JE; Wakefield D; McNeil HP, 2005, 'Mast cell accumulation and cytokine expression in the tight skin mouse model of scleroderma', Experimental Dermatology, vol. 14, pp. 295 - 302, http://dx.doi.org/10.1111/j.0906-6705.2005.00315.x

Li M; Liu K; Michalicek J; Angus JA; Hunt JE; Dell'Italia LJ; Feneley MP; Graham RM; Husain A, 2004, 'Involvement of chymase-mediated angiotensin II generation in blood pressure regulation.', J Clin Invest, vol. 114, pp. 112 - 120, http://dx.doi.org/10.1172/JCI20805

Wang H-W; McNeil HP; Husain A; Liu K; Tedla N; Thomas PS; Raftery M; King GC; Cai ZY; Hunt JE, 2002, 'Delta tryptase is expressed in multiple human tissues, and a recombinant form has proteolytic activity.', J Immunol, vol. 169, pp. 5145 - 5152, http://www.ncbi.nlm.nih.gov/pubmed/12391231

Friend DS; Gurish MF; Austen KF; Hunt J; Stevens RL, 2000, 'Senescent jejunal mast cells and eosinophils in the mouse preferentially translocate to the spleen and draining lymph node, respectively, during the recovery phase of helminth infection.', J Immunol, vol. 165, pp. 344 - 352, http://www.ncbi.nlm.nih.gov/pubmed/10861071

Huang C; Li L; Krilis SA; Chanasyk K; Tang Y; Li Z; Hunt JE; Stevens RL, 1999, 'Human Tryptases α and β/II Are Functionally Distinct Due, in Part, to a Single Amino Acid Difference in One of the Surface Loops That Forms the Substrate-binding Cleft', Journal of Biological Chemistry, vol. 274, pp. 19670 - 19676, http://dx.doi.org/10.1074/jbc.274.28.19670

Friend DS; Gurish MF; Hunt J; Hu X; Austen KF; Stevens RL, 1998, 'Fate of jejunal mast cells in mice infected with trichinella spiralis', FASEB Journal, vol. 12

Friend DS; Ghildyal N; Gurish MF; Hunt J; Hu XZ; Austen KF; Stevens RL, 1998, 'Reversible expression of tryptases and chymases in the jejunal mast cells of mice infected with Trichinella spiralis', JOURNAL OF IMMUNOLOGY, vol. 160, pp. 5537 - 5545, http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000073735400045&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a

Huang C; Friend DS; Qiu WT; Wong GW; Morales G; Hunt J; Stevens RL, 1998, 'Induction of a selective and persistent extravasation of neutrophils into the peritoneal cavity by tryptase mouse mast cell protease 6.', J Immunol, vol. 160, pp. 1910 - 1919, http://www.ncbi.nlm.nih.gov/pubmed/9469453

Hunt JE; Friend DS; Gurish MF; Feyfant E; Šali A; Huang C; Ghildyal N; Stechschulte S; Austen KF; Stevens RL, 1997, 'Mouse Mast Cell Protease 9, a Novel Member of the Chromosome 14 Family of Serine Proteases that is Selectively Expressed in Uterine Mast Cells', Journal of Biological Chemistry, vol. 272, pp. 29158 - 29166, http://dx.doi.org/10.1074/jbc.272.46.29158

Stevens RL; Oui D; McNeil HP; Friend DS; Hunt JE; Austen KF; Zhang J, 1996, 'Transgenic mice that possess a disrupted mast cell protease 5 (MMCP-5) gene can not store carboxypeptidase a (MMC-CPA) protein in their granules', FASEB Journal, vol. 10

Hunt JE; Stevens RL; Austen KF; Zhang J; Xia Z; Ghildyal N, 1996, 'Natural Disruption of the Mouse Mast Cell Protease 7 Gene in the C57BL/6 Mouse', Journal of Biological Chemistry, vol. 271, pp. 2851 - 2855, http://dx.doi.org/10.1074/jbc.271.5.2851

Bingham CO; Murakami M; Fujishima H; Hunt JE; Austen KF; Arm JP, 1996, 'A Heparin-sensitive Phospholipase A 2 and Prostaglandin Endoperoxide Synthase-2 Are Functionally Linked in the Delayed Phase of Prostaglandin D 2 Generation in Mouse Bone Marrow-derived Mast Cells', Journal of Biological Chemistry, vol. 271, pp. 25936 - 25944, http://dx.doi.org/10.1074/jbc.271.42.25936

Kandiah DA; Hunt JE; Krilis SA, 1995, 'Epitope mapping studies of antiphospholipid antibodies and β2-GPI using synthetic peptides', Lupus, vol. 4, http://dx.doi.org/10.1177/096120339400400103

Kandiah DA; Hunt JE; Krilis SA, 1995, 'Epitope mapping studies of antiphospholipid antibodies and β2-GPI using synthetic peptides', Lupus, vol. 4, pp. S7 - S11, http://dx.doi.org/10.1177/096120339400400103

Stevens JM; Hunt JE; Seymour AE; Krilis SA; Pugsley DJ, 1994, 'Sneddon's syndrome, anti-cardiolipin antibody and glomerular thrombosis', Clinical Nephrology, vol. 41, pp. 18 - 22

Hunt J; Krilis S, 1994, 'The fifth domain of β2-glycoprotein I contains a phospholipid binding site (Cys281-Cys288) and a region recognized by anticardiolipin antibodies', Journal of Immunology, vol. 152, pp. 653 - 659

Hunt JE; Adelstein S; Krilis SA, 1994, 'New basic aspects of the antiphospholipid syndrome', Clinical and Experimental Rheumatology, vol. 12, pp. 661 - 668

Hunt JE; Krilis SA, 1993, 'Antiphospholipid antibodies, beta 2-glycoprotein I and thrombosis.', Lupus, vol. 2, pp. 285 - 287, http://dx.doi.org/10.1177/096120339300200501

Vlachoyiannopoulos PG; Beigbeder G; Dueymes M; Youinou P; Hunt JE; Krilis SA; Moutsopoulos HM, 1993, 'Antibodies to Phosphatidylethanolamine in Antiphospholipid Syndrome and Systemic Lupus Erythematosus: Their Correlation with Anticardiolipin Antibodies and P 2 Glycoprotein-I Plasma Levels', Autoimmunity, vol. 16, pp. 245 - 249, http://dx.doi.org/10.3109/08916939309014642

Hunt JE; Simpson RJ; Krilis SA, 1993, 'Identification of a region of β2-glycoprotein I critical for lipid binding and anti-cardiolipin antibody cofactor activity', Proceedings of the National Academy of Sciences of the United States of America, vol. 90, pp. 2141 - 2145

VLACHOYIANNOPOULOS PG; KRILIS SA; HUNT JE; MANOUSSAKIS MN; MOUTSOPOULOS HM, 1992, 'Patients with anticardiolipin antibodies with and without antiphospholipid syndrome: their clinical features and β 2 -glycoprotein-l plasma levels', European Journal of Clinical Investigation, vol. 22, pp. 482 - 487, http://dx.doi.org/10.1111/j.1365-2362.1992.tb01494.x

McNeil HP; Hunt JE; Krilis SA, 1992, 'Antiphospholipid antibodies - New insights into their specificity and clinical importance', Scandinavian Journal of Immunology, vol. 36, pp. 647 - 652, http://dx.doi.org/10.1111/j.1365-3083.1992.tb03124.x

Hunt JE; McNeil HP; Morgan GJ; Crameri RM; Krilis SA, 1992, 'A phospholipid-beta 2-glycoprotein I complex is an antigen for anticardiolipin antibodies occurring in autoimmune disease but not with infection.', Lupus, vol. 1, pp. 75 - 81

HUNT JE; MCNEIL HP; KRILIS SA, 1991, 'ANTICARDIOLIPIN ANTIBODIES FROM AUTOIMMUNE-DISEASE AND INFECTION HAVE DIFFERENT BINDING SPECIFICITIES', FASEB JOURNAL, vol. 5, pp. A1088 - A1088, http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1991FC55000993&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a

Ang C; Clark E; Young I; Owe-Young R; Eyland A; Hunt J; Chesterman C; Krilis S, 1990, '15-Hydroxyeicosatetraenoic acid synthesis from exogenous arachidonate by bronchial mucosa', Journal of Lipid Mediators, vol. 2, pp. 263 - 269

McNeil HP; Hunt JE; Krilis SA, 1990, 'New aspects of anticardiolipin antibodies', Clinical and Experimental Rheumatology, vol. 8, pp. 525 - 527

Conference Papers

Wang HW; Tedia N; Hunt JE; Wakefield D; McNeill HP, 2000, 'Mechanisms of mast cell accumulation in tight skin (Tsk) mouse model of scleroderma', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, pp. A1151 - A1151, http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000086643101404&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a